Hormonal Signaling during dPCD: Cytokinin as the Determinant of RNase-Based Self-Incompatibility in Solanaceae

نویسندگان

چکیده

Research into molecular mechanisms of self-incompatibility (SI) in plants can be observed representatives various families, including Solanaceae. Earlier studies the S-RNase-based SI petunia (Petunia hybrida E. Vilm.) demonstrate that programmed cell death (PCD) is an factor. These suggest phytohormon cytokinin (CK) putative activator caspase-like proteases (CLPs). In this work, data confirming hypothesis were obtained two model objects—petunia and tomato (six Solanaceae representatives). The exogenous zeatin treatment stigmas before a compatible pollination activates CLPs pollen tubes vivo, as shown via intravital imaging CLP activities. CK at any concentration slows down germination growth male gametophytes both vitro vivo; shifts pH cytoplasm (PHc) to acid region, thereby creating optimal conditions for function inhibiting F-actin formation and/or destructing cytoskeleton point foci during SI-induced PCD; accumulates style tissues response. activity ISOPENTENYLTRANSFERASE 5 (IPT5) gene moment exceeds its cross-compatible pollination, levels expression CKX1 CKX2 genes (CK OXIDASE/DEHYDROGENASE) are significantly lower self-incompatible pollination. All suggests plays decisive role mechanism underlying PCD.

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ژورنال

عنوان ژورنال: Biomolecules

سال: 2023

ISSN: ['2218-273X']

DOI: https://doi.org/10.3390/biom13071033